Home » » The gene targets for the treatment of Mesothelioma

The gene targets for the treatment of Mesothelioma

suex | 0 komentar
Reading Always - The gene targets for the treatment of Mesothelioma, Several similar genes are thought to help prediction of mesothelioma. Patients will do my best, after selsai surgery. It may also be seagai a good target for gene therapy, for people who suffer from this disease. This conclusion is given by a physician for this disease mesothelioma. Dr. David Sugarbaker, and co-workers who participated helped to investigate and provide conclusions about the disease.

Mesothelioma is highly resistant to standard treatments.  Gene therapy, which harnesses and uses the body’s own nature defense system, is being studied as a promising alternative.

Starting with lung tissue from both healthy patients and those with malignant pleural mesothelioma, the researchers used a high-tech RNA inhibition screen to knockdown gene expression levels of the four genes in the test: ARHGDIA, COBLL1, PKM2 and TM4SF1. They then used a test called a quantitative RT-PCR to ensure that they had, in fact, inhibited expression of the selected genes in the cell samples.

The next step was to watch for changes in the rate of cellular death (apoptosis) and/or replication (mitosis).  For this, the researchers used immunological assays and video-assisted microscopy.  They also recorded changes in the shape and size of each cell’s nucleus.  Each complete experiment was conducted three times to ensure accurate results.

The results revealed some vital information about the role of the selected genes in mesothelioma.  When the ARHGDIA, COBLL1, and TM4SF1 genes were knocked down, there was a 2- to 4-fold increased in cellular death in the tumor cells.  In normal cells, only the loss of the ARHGDIA gene triggered apoptosis.  The PKM2 gene did not appear to affect apoptosis in either the normal cells or the tumor cells and none of the cells seemed to have any effect on mitosis.

But the fact that three of the genes tested had a clear connection to apoptosis could make them prime targets for drugs that regulate their expression in mesothelioma patients.  In an article on the study in the journal BMC Cancer, the researchers conclude, “These genes, and their related intracellular signaling pathways, may represent potential therapeutic targets in mesothelioma. 

Share this article :

0 komentar:

Post a Comment

Online Marketing
Add blog to our blog directory.
Support : Cretor | Blogger Unik
Copyright © 2011. Reading Always - All Rights Reserved